Targeted drug delivery and controlled drug release depend on specific polymers. Polymer nanoparticles are therefore typically used for encapsulation or as matrix for coupling or adhesion of drug molecules.  Due to their biocompatibility, biodegradability and possibility of functionalization, particles made of recombinant spider silk proteins are promising candidates for such applications.  They have previously been confirmed as suitable drug and gene carrier. Various drug substances or biologicals could be loaded, and steady release was observed over a defined period. [3, 4] However, the release of loaded substances could not be controlled spatio-temporally, since they were bound non-covalently via charge-charge or hydrophobic interactions. Here, we extended the nanoparticles platform made of recombinant spider silk proteins. We present chemically and genetically modified variants of the spider silk protein eADF4(C16) facilitating a triggered release upon physiological changes in pH or redox state. Chemical modification of the proteins’ carboxyl groups with a hydrazone linker followed by coupling of model drugs led to a pH-responsive carrier system, while coupling of model drugs to cysteine containing variants via disulfide bonds resulted in carriers for a redox-responsive release. Release was quantified UV-Vis spectroscopically and additionally, cell culture experiments were performed using fluorescent-labeled compounds confirming intracellular release.