Wrinkles are a natural effect that can be observed both macroscopically on folding mountains and microscopically on human skin or on plant surfaces. The wrinkle formation is based on a mechanical mismatch between a stiff hard layer fixed on top of an elastomeric substrate. With controlled strain conditions well-ordered periodic deformation becomes possible, in which wavelength and amplitude depends on the mechanical properties of layer and substrate . This allows selective and localized wrinkling, which can be used to create hierarchical structures with precisely located line defects. Number and position of these line defects highly depend on the process conditions, under which the waves have been produced. We therefore demonstrate a method with precise control via substrate stiffness and layer property control in a low pressure plasma process. With this controlled and localized surface wrinkling, functionalized surfaces for biological approaches can be obtained e.g. control of bacteria adhesion .
 B. A. Glatz, M. Tebbe, B. Kaoui, R. Aichele, C. Kuttner, A. E. Schedl, H.-W. Schmidt, W. Zimmermann and A. Fery: Hierarchical line-defect patterns in wrinkled surfaces, 2015, Soft Matter, 11, 3332 – 3339
 Helbig, R., et al: The impact of structure dimensions on initial bacterial adhesion, 2016, Biomater Sci 4(7): 1074-1078.