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Special Poster Session Biofabrication

4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers

Tuesday (17.03.2020)
18:22 - 18:25
Part of:
Line-Up:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication


Majority of human body mass (40%) consists of skeletal muscle. Although regeneration rate of skeletal muscle is relatively high, they cannot heal in the case of tumor ablation or severe damage. Skeletal muscle tissue has a highly complex tubular structure that can be mimicked using tubular self-folding polymeric films [1]. However, high porosity is important for easier transport of nutrient and waste products and can lead to improved cell growth and differentiation. Self-folding electrospun bilayers with high porosity would provide a suitable substrate for tissue growth in a controllable tubular shape [2, 3].

We fabricated electrospun bilayer mats consisting of a layer of aligned polycaprolactone (PCL) fibers, that could guide myoblast cell growth in preferable orientation, and a layer of anisotropic methacrylated alginate fiber (AA-MA). PCL/AA-MA bilayer mats undergo shape-transformation in contact with aqueous medium, a process that is depending on the overall thickness of bilayer, the thickness of each layer and the geometry of the mat. Proper selection of these parameters allowed fabrication of scroll-like tubes for encapsulation of myoblasts. Using fabricated bilayer mats, we were able to align myoblasts along the axis of the oriented PCL fibers. C2C12 myoblasts cells were further differentiated into aligned myotubes that were contractible under electrical stimulation [3].


1. Vannozzi L; Yasa C.I; Ceylan H; Menciassi A; Ricotti L; Sitti M; Macromol. Biosci., 2018, 18, 1700377.

2. Apsite I; Stoychev G; Zhang W; Jehnichen D; Xie J; Ionov L; Biomacromolecules, 2017, 18, 3178.

3. Apsite I; Uribe J.M; Posada A.F; Rosenfeldt S; Salehi S; Ionov L; Biofabrication, 2019, DOI:10.1088/1758-5090/ab4cc4




 

Speaker:
Indra Apsite
University of Bayreuth
Additional Authors:
  • Dr. Sahar Salehi
    University of Bayreuth
  • Prof. Leonid Ionov
    University of Bayreuth