Programme

Unfurtunately the monastery Irsee has been closed by the Bavarian government due to the corona virus.

Therefore we have decided to offer the conference as a web conference only.

In those unpredictable times, protecting our participants’ health has the highest priority for us!

The scientific exchange must not come to a complete standstill and we believe that with this web solution we have found a way to keep the scientific community going.

In order to participate in the livestream of the conference as easy as possible and to give your presentation, we recommend the use of the web browsers Google Chrome or Microsoft Edge.

Here you can find detailed instructions for using the livestream.

As a participant of the Bioinspired 2020 you have received an e-mail containing the login data for the web conference.

The login data for the sessions are provided daily.

In order to allow all conference participants access to the posters, we ask you to upload your poster by login on the conference homepage and clicking on the button "My Submission" in the upper right corner of the conference homepage. Then select the submission to which you want to upload the poster and upload the poster at the bottom of the page.

Furthermore we would like to ask all poster authors to prepare 4 PowerPoint slides to present your poster to the audience.
Please also include your contact details on the digital poster to allow participants to reach you with questions.

Please note that your poster will be pictured publically.

As poster author you can upload your poster similar to the way you submitted your abstract.
Click on "My Submissions" in the upper right corner at the homepage.

Poster documents can be found by opening the respective abstract in the online programme.
If a poster document has already been uploaded for the abstract, it can then be opened and downloaded.

Please note that your poster will be pictured publically.

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Please use for this the Q&A (F&A) button!

For further scientific exchange we implemented a discussion forum on the homepage of the DGM.
Please visit discussion.dgm.de and use your DGM or Bioinspired user credentials to login.

Back to overview

Special Poster Session Biofabrication

Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells

Tuesday (17.03.2020)
17:40 - 17:43

Hyaluronic acid (HA) represents a desirable material for cartilage biofabrication approaches since it is one of the major components of the cartilage extracellular matrix (ECM), where it provides not only structural and mechanical support for mesenchymal stem cells (MSCs) but also functions as a signaling molecule. This study aims to develop a flexible hydrogel platform for 3D bioprinting of MSCs by a dual-stage crosslinking process based on thiol-modified HA (HASH) and two different crosslinkers (acryl- and allyl-modified PEG).

The chemical modifications of all three components were established and optimized for gram scale production. A 3D printable formulation of HASH and Acryl-PEG, that partially crosslinks via Michael-Addition in stage 1, was identified by a custom-made screening method. Thereby, a fixed amount of HASH was combined with different amounts of Acryl-PEG in PBS, incubated at 37°C for 1h and the resulting hydrogels were analyzed with respect to their printability and properties. In stage 2, a thiol-ene reaction was conducted with Allyl-PEG under UV-light in the presence of I2959 to increase shape fidelity and biomechanical properties of the 3D bioprinted construct. Therefore, a swelling test of 3D printed hydrogels in PBS with different amounts of PEG-Allyl was performed to identify a volume stable formulation. With the final formulation, MSCs were 3D bioprinted in two stacked layers and the initial cell survival was analyzed by live-dead staining.

The study demonstrated the development of an ink formulation of HASH, Acryl-PEG and Allyl-PEG with tunable properties and a polymer content below 6 (w/v) %, suitable for 3D printing. With the developed ink it was possible to 3D bioprint MSCs with good cell survival. Ongoing work focuses on long-term survival and chondrogenic differentiation of MSCs.

 

Acknowledgment

This project is funded by the „Deutsche Forschungsgemeinschaft“ (DFG, German Research Foundation) project number 326998133 - TRR-225 (subproject A02).

Speaker:
Leonard Forster
University Hospital Würzburg
Additional Authors:
  • Julia Hauptstein
    University of Würzburg
  • Prof. Dr. Torsten Blunk
    University of Würzburg
  • Dr. Jörg Teßmar
    University of Würzburg
  • Prof. Dr. Jürgen Groll
    University of Würzburg

Dateien

Category Short file description File description File Size
Poster Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells I feel very uncomfortable to upload unpublished results on a website where everyone can download them without my notice. This is a pixelated version of my poster. More details can be requested per email. I ask for your understanding. 17 MB Download