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Special Poster Session Biofabrication

Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells

Tuesday (17.03.2020)
17:40 - 17:43
Part of:
Line-Up:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication


Hyaluronic acid (HA) represents a desirable material for cartilage biofabrication approaches since it is one of the major components of the cartilage extracellular matrix (ECM), where it provides not only structural and mechanical support for mesenchymal stem cells (MSCs) but also functions as a signaling molecule. This study aims to develop a flexible hydrogel platform for 3D bioprinting of MSCs by a dual-stage crosslinking process based on thiol-modified HA (HASH) and two different crosslinkers (acryl- and allyl-modified PEG).

The chemical modifications of all three components were established and optimized for gram scale production. A 3D printable formulation of HASH and Acryl-PEG, that partially crosslinks via Michael-Addition in stage 1, was identified by a custom-made screening method. Thereby, a fixed amount of HASH was combined with different amounts of Acryl-PEG in PBS, incubated at 37°C for 1h and the resulting hydrogels were analyzed with respect to their printability and properties. In stage 2, a thiol-ene reaction was conducted with Allyl-PEG under UV-light in the presence of I2959 to increase shape fidelity and biomechanical properties of the 3D bioprinted construct. Therefore, a swelling test of 3D printed hydrogels in PBS with different amounts of PEG-Allyl was performed to identify a volume stable formulation. With the final formulation, MSCs were 3D bioprinted in two stacked layers and the initial cell survival was analyzed by live-dead staining.

The study demonstrated the development of an ink formulation of HASH, Acryl-PEG and Allyl-PEG with tunable properties and a polymer content below 6 (w/v) %, suitable for 3D printing. With the developed ink it was possible to 3D bioprint MSCs with good cell survival. Ongoing work focuses on long-term survival and chondrogenic differentiation of MSCs.


Acknowledgment

This project is funded by the „Deutsche Forschungsgemeinschaft“ (DFG, German Research Foundation) project number 326998133 - TRR-225 (subproject A02).

Speaker:
Leonard Forster
University Hospital Würzburg
Additional Authors:
  • Julia Hauptstein
    University of Würzburg
  • Prof. Dr. Torsten Blunk
    University of Würzburg
  • Dr. Jörg Teßmar
    University of Würzburg
  • Prof. Dr. Jürgen Groll
    University of Würzburg