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Special Poster Session Biofabrication

Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks

Tuesday (17.03.2020)
17:43 - 17:46
Part of:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication

Bioprinting of larger tissues and eventually organs presents a tremendous opportunity to challenge the status quo for medical treatments of all kinds of illnesses. Necessary for the successful implementation of bioprinting is improving the cell survival during the printing process, where cells are subjected to significant shear forces. A possible way to improve cell viability and to expand the bioprinting window is the encapsulation of cells in hydrogels, which can be done using microfluidics[1].

Poly(2-oxazolines) possess unique properties making them an ideal synthetic polymer for the stable encapsulation of cells. They are biocompatible[2], while providing excellent opportunities for a functionalization at the side chain and both termini of the polymer. Several different modifications, for example free thiol groups at the side chain, are available which allow covalent crosslinking via thiol-ene reaction[3] or Michael addition[4]. To obtain POx-based hydrogel precursors 2-ethyl-2-oxazoline was randomly copolymerized with 2-(3-butenyl)-2-oxazoline, which were then functionalized with free thiol groups for hydrogel formation via thiol-ene reaction. Combining these two polymers with double three-dimensional flow focusing microfluidic chips allows the production of droplets of varying sizes with high uniformity. The first 3D channel intersection can be used for flow focusing and/or mixing of A/B components, while the second 3D intersection is used for stable droplet production. A 3D contoured narrowing allows for precise control of achieved droplet sizes. After production, the droplets can be crosslinked on demand either using UV irradiation or visible light, depending on the chosen radical initiator. This allows to tailor the system towards specific needs of cells as well as further production parameters. The final droplets can then be used either as bioink alone or as additive in conventional bioinks to ensure cellular protection.

[1] E. Tumarkin, E. Kumacheva, Chem. Soc. Rev. 2009, 38, 2161.

[2] B. Verbraeken, B. D. Monnery, K. Lava, R. Hoogenboom, Eur. Polym. J. 2017, 88, 451.

[3] J. Blöhbaum, I. Paulus, A. Pöppler, J. Tessmar, J. Groll, J. Mater. Chem. B. 2019, 7, 178.

[4] D. P. Nair, M. Podgórski, S. Chatani, T. Gong, W. Xi, C. R. Fenoli, C. N. Bowman, Chem. Mater. 2014, 26, 724.

This research has received funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – project number 326998133 – TRR 225 (subproject A06).

Ilona Paulus
University Hospital Würzburg
Additional Authors:
  • Benjamin Reineke
    Forschungszentrum Jülich GmbH
  • Dr. Stephan Hauschild
    Forschungszentrum Jülich GmbH
  • Prof. Dr. Stephan Förster
    Forschungszentrum Jülich GmbH
  • Prof. Dr. Jürgen Groll
    University of Würzburg