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Special Poster Session Biofabrication

Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations

Tuesday (17.03.2020)
17:46 - 17:49
Part of:
Line-Up:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication


Over the last years, bioink development was one of the key aspects in the interdisciplinary field of biofabrication, especially for dispense plotting. Natural derived biopolymers are preferred as bioinks due to their often good cytocompatibility.[1] However, in various applications scenarios, biopolymers also have limitations, such as suboptimal viscoelastic properties. Arguably, synthetic polymers can be tuned more easily for specific applications, in particular platforms that allow fine-tuning of physicochemical and viscoelastic properties are favourable. Poly(2-oxazoline)s are widely discussed as biomaterials in literature.[2] Lorson et al. established a thermogelling (G´ ~ 4 kPa) and printable poly(2-oxazoline)/poly(2-oxazine) (POx/POzi) supramolecular hydrogel with defined, reversible and fast sol/gel transition.[3] We recently investigated the inverse thermogelation properties of aqueous ABA triblock copolymers based on POx/POzi solutions into shear thinning and printable inks that feature a very large range of storage moduli (G´=0.1-110 kPa) and sol/gel transitions depending on polymer concentration and composition. Using these platforms as support materials in hybrid systems and support bath approaches during 3D bioprinting we demonstrate the advantages of different thermogelling structures. For advanced utilization of the described synthetic platforms as functional bioinks, two major limitations, namely long-term stability and biofunctionalization, have to be addressed. To introduce cell recognition peptide sequences and chemical crosslinking to ensure temporal mechanical control via Diels-Alder reaction, we adapted the well-established post polymerization modification (PPM) approach,[4] and examined the influence of modification and crosslinking regarding printability, cross-linking kinetics and swelling followed by first long-term cell culture experiments using RGD-sequences as cell adhesion motives to ensure cell attachment of NIH 3T3 cells. In summary, we developed different physical hydrogels as support materials for bioprinting. Furthermore, via PPM we could overcome limitations regarding long term stability and bio-functionality to obtain a versatile synthetic bioink platform, applicable in many applications in the field of biofabrication.



 

Speaker:
Lukas Hahn
University of Würzburg
Additional Authors:
  • Matthias Beudert
    University of Würzburg
  • Dr. Thomas Lorson
    University of Würzburg
  • Dr. Tessa Lühmann
    University of Würzburg
  • Prof. Dr. Robert Luxenhofer
    University of Würzburg