Programme

Unfurtunately the monastery Irsee has been closed by the Bavarian government due to the corona virus.

Therefore we have decided to offer the conference as a web conference only.

In those unpredictable times, protecting our participants’ health has the highest priority for us!

The scientific exchange must not come to a complete standstill and we believe that with this web solution we have found a way to keep the scientific community going.

In order to participate in the livestream of the conference as easy as possible and to give your presentation, we recommend the use of the web browsers Google Chrome or Microsoft Edge.

Here you can find detailed instructions for using the livestream.

As a participant of the Bioinspired 2020 you have received an e-mail containing the login data for the web conference.

The login data for the sessions are provided daily.

In order to allow all conference participants access to the posters, we ask you to upload your poster by login on the conference homepage and clicking on the button "My Submission" in the upper right corner of the conference homepage. Then select the submission to which you want to upload the poster and upload the poster at the bottom of the page.

Furthermore we would like to ask all poster authors to prepare 4 PowerPoint slides to present your poster to the audience.
Please also include your contact details on the digital poster to allow participants to reach you with questions.

Please note that your poster will be pictured publically.

As poster author you can upload your poster similar to the way you submitted your abstract.
Click on "My Submissions" in the upper right corner at the homepage.

Poster documents can be found by opening the respective abstract in the online programme.
If a poster document has already been uploaded for the abstract, it can then be opened and downloaded.

Please note that your poster will be pictured publically.

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Please use for this the Q&A (F&A) button!

For further scientific exchange we implemented a discussion forum on the homepage of the DGM.
Please visit discussion.dgm.de and use your DGM or Bioinspired user credentials to login.

Back to overview

Special Poster Session Biofabrication

Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes

Tuesday (17.03.2020)
17:49 - 17:52

Introduction:

3D bioprinting is a promising and innovative technique in the field of tissue engineering allowing the generation of highly precise constructs for different purposes. During that process, cells are facing different challenges to survive in the hydrogel environment. Our project investigates shear stress impact as well as adherence behavior of human mesenchymal stromal cells (hMSC) in biofabrication processes and aims to enhance the glycocalyx mediated cell stability and the lectin ligand mediated adhesion in bioinks by applying glycoengineering.

 

Experimental Methods:

For establishing metabolic glycoengineering, hMSC were first incubated with different azido sugars for 48 h followed by click-reactions in which cells were either incubated with DBCO-Cy3 as click molecule for 1 h or in presence of Cu ions with alkyne-Cy3 for 5 min. The fluorescence of the resulting dye-sugar complex was microscopically evaluated over time. For galectin-1 binding studies, cells were incubated with an artificial galectin-1 ligand or seeded onto a ligand coated glass slide.

 

Results:

The azido sugar expression in the glycocalyx could be microscopically detected up to 48 h identifiying the mannosamine variant as superior regarding cell viability and incorporation efficiency. The glycochip assay resulted in the appearance of non-adherent cell spheroids, but revealed no cell adhesion toward the galectin-1 ligand coated regions. A first galectin-1 gene expression analysis showed no remarkably altered mRNA level after incubation with the ligand.

 

Conclusion:

Since the metabolic glycoengineering is working, suitable molecules can now be identified to be introduced into the glycocalyx and evaluated for cell rigidity-increasing effects before and after 3D bioprinting. To support data interpretation, the shear stress impact on cell features after printing will be characterized before with unmodified cells. The adherence assay needs to be redesigned since cell adhesion was expected and the glycochip basic functionalization might impede surface-cell interaction. The galectin-1 ligand impact on cell functions will be further characterized.

Speaker:
Stephan Altmann
University of Würzburg
Additional Authors:
  • Jürgen Mut
    University of Würzburg
  • Natalia Wolf
    University of Würzburg
  • Julian Bechold
    University of Würzburg
  • Prof. Dr. Franz Jakob
    University of Würzburg
  • Prof. Dr. Jürgen Seibel
    University of Würzburg
  • Prof. Dr. Regina Ebert
    University of Würzburg