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Special Poster Session Biofabrication

Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes

Tuesday (17.03.2020)
17:49 - 17:52
Part of:
Line-Up:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication


Introduction:

3D bioprinting is a promising and innovative technique in the field of tissue engineering allowing the generation of highly precise constructs for different purposes. During that process, cells are facing different challenges to survive in the hydrogel environment. Our project investigates shear stress impact as well as adherence behavior of human mesenchymal stromal cells (hMSC) in biofabrication processes and aims to enhance the glycocalyx mediated cell stability and the lectin ligand mediated adhesion in bioinks by applying glycoengineering.


Experimental Methods:

For establishing metabolic glycoengineering, hMSC were first incubated with different azido sugars for 48 h followed by click-reactions in which cells were either incubated with DBCO-Cy3 as click molecule for 1 h or in presence of Cu ions with alkyne-Cy3 for 5 min. The fluorescence of the resulting dye-sugar complex was microscopically evaluated over time. For galectin-1 binding studies, cells were incubated with an artificial galectin-1 ligand or seeded onto a ligand coated glass slide.


Results:

The azido sugar expression in the glycocalyx could be microscopically detected up to 48 h identifiying the mannosamine variant as superior regarding cell viability and incorporation efficiency. The glycochip assay resulted in the appearance of non-adherent cell spheroids, but revealed no cell adhesion toward the galectin-1 ligand coated regions. A first galectin-1 gene expression analysis showed no remarkably altered mRNA level after incubation with the ligand.


Conclusion:

Since the metabolic glycoengineering is working, suitable molecules can now be identified to be introduced into the glycocalyx and evaluated for cell rigidity-increasing effects before and after 3D bioprinting. To support data interpretation, the shear stress impact on cell features after printing will be characterized before with unmodified cells. The adherence assay needs to be redesigned since cell adhesion was expected and the glycochip basic functionalization might impede surface-cell interaction. The galectin-1 ligand impact on cell functions will be further characterized.

 

Speaker:
Stephan Altmann
University of Würzburg
Additional Authors:
  • Jürgen Mut
    University of Würzburg
  • Natalia Wolf
    University of Würzburg
  • Julian Bechold
    University of Würzburg
  • Prof. Dr. Franz Jakob
    University of Würzburg
  • Prof. Dr. Jürgen Seibel
    University of Würzburg
  • Prof. Dr. Regina Ebert
    University of Würzburg