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Special Poster Session Biofabrication

Fiber reinforced hydrogels – a new platform technology in biofabrication

Tuesday (17.03.2020)
17:52 - 17:55
Part of:
Line-Up:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication


Hydrogels display highly attractive properties for their use in the field of biofabrication and tissue engineering. With a water content above 80%, they form 3D network structures that can resemble parts of the extracellular matrix of native tissue and are therefore predestined to be used as bioinks in 3D printing processes including living cells. They can be prepared from various polymers, either synthetic or biological, to match the requirements of the targeted tissue that must be replaced. To enable sufficient resolutions in the printing process, high polymer concentrations are generally required resulting in increased shear forces on the incorporated cells upon printing. Unfortunately, this is commonly known to be at the expense of cell viability. To simultaneously enable good printing resolutions and maintain high cell viability, it is important to trigger the rheological properties of bioinks towards low internal shear forces upon-, and high shape fidelity after printing. Filler materials such as fibers and spheres provide a widely used strategy to change rheological properties of solutions and melts and can further be applied to reinforce matrix materials. In this work, we developed a systematic approach to adapt the flow behavior of bioinks upon printing and increase the mechanical performance of the printed constructs. Electrospinning was applied to produce micro-fiber fragments made from poly-ε-caprolactone (PCL), providing good mechanical properties and biodegradability. Thermo-responsive gelatin methacrylate (GelMa) hydrogels were used as the matrix material promoting cell adhesion and proliferation. Pluronic was applied as an additional test system to systematically investigate the influence of fragment concentration in a shear thinning system. The resulting composite systems should provide adjustable rheological properties that can be triggered by particle size and filling density. This systematic study includes electrospinning of PCL fiber fragments and SEM analysis thereof, surface modification of PCL particles by plasma treatment and oscillatory rheology to determine the effects of size, concentration and surface modification on the rheological properties of the resulting composite hydrogels.

Speaker:
Dipl.-Ing. David Sonnleitner
University of Bayreuth
Additional Authors:
  • Prof. Dr. Gregor Lang
    University of Bayreuth