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Special Poster Session Biofabrication

3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks

Tuesday (17.03.2020)
17:55 - 17:58
Part of:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication

In biofabrication, 3D multicellular spheroids represent attractive building blocks for tissue constructs in regenerative medicine and in the development of disease models. However, the use of spheroids in 3D bioprinting approaches as components of bioinks is associated with major challenges regarding the printing process impacting on spheroid distribution and cell viability. Therefore, in this study, employing 3D spheroids made from human adipose-derived stromal cells (ASC), we established a suitable bioink composition and determined favorable processing parameters enabling 3D bioprinting. Furthermore, adipogenic differentiation and cytokine secretion of ASC within the spheroids were evaluated after printing.

ASC spheroids with different cell numbers were generated using a micro-mold system facilitating large-scale production. Spheroids composed of 2500 cells (diameter 220 µm) were suspended in a hydrogel solution consisting of thiol-functionalized hyaluronic acid and allyl-modified polyglycidol for UV crosslinking. The addition of 1 wt.% unmodified high molecular weight HA (hmHA) increased bioink viscosity enabling bioprinting. Furthermore, supplementation with hmHA decisively decreased spheroid sedimentation during printing and UV crosslinking resulting in a distinctly more homogeneous spheroid distribution in the final constructs. With regard to printing process parameters, stainless steel precision needles with an inner diameter of 330 μm, and a pressure of 1 bar proved to be most suitable for spheroid printing, as shown by viability and metabolic assays. Adipogenic differentiation of ASC was demonstrated over 21 days in cast and printed constructs, with distinctly higher amounts of synthesized triglycerides and strongly increased mRNA expression (qRT-PCR) of marker genes such as PPARG and aP2, as compared to undifferentiated constructs. Regarding cytokine secretion, also clear differences were detected between supernatants of undifferentiated (e.g., higher IL-6 levels) and differentiated ASC (e.g., higher adiponectin levels). Under favorable processing conditions, with regard to cell viability, differentiation capacity, and secretion behavior, no differences between printed and cast constructs were observed. The study facilitates the future use of multicellular spheroids as building blocks in 3D bioprinting, e.g., ASC spheroids may be utilized in disease model development as an important stromal component in 3D models of breast cancer.


Hannes Horder
University Hospital Würzburg
Additional Authors:
  • Mar Guaza Lasheras
    University of Würzburg
  • Nadine Grummel
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Ben Fabry
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Dr. Jörg Teßmar
    University of Würzburg
  • Dr. Petra Bauer-Kreisel
    University of Würzburg
  • Prof. Dr. Torsten Blunk
    University of Würzburg