Programme

Unfurtunately the monastery Irsee has been closed by the Bavarian government due to the corona virus.

Therefore we have decided to offer the conference as a web conference only.

In those unpredictable times, protecting our participants’ health has the highest priority for us!

The scientific exchange must not come to a complete standstill and we believe that with this web solution we have found a way to keep the scientific community going.

In order to participate in the livestream of the conference as easy as possible and to give your presentation, we recommend the use of the web browsers Google Chrome or Microsoft Edge.

Here you can find detailed instructions for using the livestream.

As a participant of the Bioinspired 2020 you have received an e-mail containing the login data for the web conference.

The login data for the sessions are provided daily.

In order to allow all conference participants access to the posters, we ask you to upload your poster by login on the conference homepage and clicking on the button "My Submission" in the upper right corner of the conference homepage. Then select the submission to which you want to upload the poster and upload the poster at the bottom of the page.

Furthermore we would like to ask all poster authors to prepare 4 PowerPoint slides to present your poster to the audience.
Please also include your contact details on the digital poster to allow participants to reach you with questions.

Please note that your poster will be pictured publically.

As poster author you can upload your poster similar to the way you submitted your abstract.
Click on "My Submissions" in the upper right corner at the homepage.

Poster documents can be found by opening the respective abstract in the online programme.
If a poster document has already been uploaded for the abstract, it can then be opened and downloaded.

Please note that your poster will be pictured publically.

You can ask your questions via chat already during the presentations!
Please use for this the Q&A (F&A) button!

For further scientific exchange we implemented a discussion forum on the homepage of the DGM.
Please visit discussion.dgm.de and use your DGM or Bioinspired user credentials to login.

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Special Poster Session Biofabrication

3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks

Tuesday (17.03.2020)
17:55 - 17:58

In biofabrication, 3D multicellular spheroids represent attractive building blocks for tissue constructs in regenerative medicine and in the development of disease models. However, the use of spheroids in 3D bioprinting approaches as components of bioinks is associated with major challenges regarding the printing process impacting on spheroid distribution and cell viability. Therefore, in this study, employing 3D spheroids made from human adipose-derived stromal cells (ASC), we established a suitable bioink composition and determined favorable processing parameters enabling 3D bioprinting. Furthermore, adipogenic differentiation and cytokine secretion of ASC within the spheroids were evaluated after printing.

ASC spheroids with different cell numbers were generated using a micro-mold system facilitating large-scale production. Spheroids composed of 2500 cells (diameter 220 µm) were suspended in a hydrogel solution consisting of thiol-functionalized hyaluronic acid and allyl-modified polyglycidol for UV crosslinking. The addition of 1 wt.% unmodified high molecular weight HA (hmHA) increased bioink viscosity enabling bioprinting. Furthermore, supplementation with hmHA decisively decreased spheroid sedimentation during printing and UV crosslinking resulting in a distinctly more homogeneous spheroid distribution in the final constructs. With regard to printing process parameters, stainless steel precision needles with an inner diameter of 330 μm, and a pressure of 1 bar proved to be most suitable for spheroid printing, as shown by viability and metabolic assays. Adipogenic differentiation of ASC was demonstrated over 21 days in cast and printed constructs, with distinctly higher amounts of synthesized triglycerides and strongly increased mRNA expression (qRT-PCR) of marker genes such as PPARG and aP2, as compared to undifferentiated constructs. Regarding cytokine secretion, also clear differences were detected between supernatants of undifferentiated (e.g., higher IL-6 levels) and differentiated ASC (e.g., higher adiponectin levels). Under favorable processing conditions, with regard to cell viability, differentiation capacity, and secretion behavior, no differences between printed and cast constructs were observed. The study facilitates the future use of multicellular spheroids as building blocks in 3D bioprinting, e.g., ASC spheroids may be utilized in disease model development as an important stromal component in 3D models of breast cancer.

Speaker:
Hannes Horder
University Hospital Würzburg
Additional Authors:
  • Mar Guaza Lasheras
    University of Würzburg
  • Nadine Grummel
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Ben Fabry
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Dr. Jörg Teßmar
    University of Würzburg
  • Dr. Petra Bauer-Kreisel
    University of Würzburg
  • Prof. Dr. Torsten Blunk
    University of Würzburg