Programme

Unfurtunately the monastery Irsee has been closed by the Bavarian government due to the corona virus.

Therefore we have decided to offer the conference as a web conference only.

In those unpredictable times, protecting our participants’ health has the highest priority for us!

The scientific exchange must not come to a complete standstill and we believe that with this web solution we have found a way to keep the scientific community going.

In order to participate in the livestream of the conference as easy as possible and to give your presentation, we recommend the use of the web browsers Google Chrome or Microsoft Edge.

Here you can find detailed instructions for using the livestream.

As a participant of the Bioinspired 2020 you have received an e-mail containing the login data for the web conference.

The login data for the sessions are provided daily.

In order to allow all conference participants access to the posters, we ask you to upload your poster by login on the conference homepage and clicking on the button "My Submission" in the upper right corner of the conference homepage. Then select the submission to which you want to upload the poster and upload the poster at the bottom of the page.

Furthermore we would like to ask all poster authors to prepare 4 PowerPoint slides to present your poster to the audience.
Please also include your contact details on the digital poster to allow participants to reach you with questions.

Please note that your poster will be pictured publically.

As poster author you can upload your poster similar to the way you submitted your abstract.
Click on "My Submissions" in the upper right corner at the homepage.

Poster documents can be found by opening the respective abstract in the online programme.
If a poster document has already been uploaded for the abstract, it can then be opened and downloaded.

Please note that your poster will be pictured publically.

You can ask your questions via chat already during the presentations!
Please use for this the Q&A (F&A) button!

For further scientific exchange we implemented a discussion forum on the homepage of the DGM.
Please visit discussion.dgm.de and use your DGM or Bioinspired user credentials to login.

Back to overview

Lecture

Hyaluronic Acid-based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix

Tuesday (17.03.2020)
15:50 - 16:10

Hyaluronic acid (HA) represents a main component of the human extracellular matrix (ECM) and is, hence, a desirable material in biofabrication. For cartilage regeneration, in principle, HA is an attractive bioink, however, homogeneous ECM distribution can represent a major challenge due to high polymer contents enabling bioprinting. Therefore, in this study, HA-based bioink formulations were investigated that allowed for reduction of polymer content, and ECM development was evaluated after chondrogenic differentiation of human mesenchymal stromal cells (MSC).

In order to form a range of stable hydrogels, thiol-modified HA (HA-SH) was UV-crosslinked with allyl-modified polyglycidol employing different concentrations of both components. MSC-laden constructs were cultured in chondrogenic medium for 21 days. Decreasing total polymer content from 15 wt.% to 3 wt.% increased construct quality by improved distribution of MSC-derived ECM within the construct, while quantitative analysis of ECM components revealed no significant differences. In order to enable PCL-supported 3D bioprinting, bioink viscosity was increased by further supplementation with 1 wt.% unmodified high molecular weight HA (hmHA). Strikingly, addition of hmHA resulted in completely homogeneous ECM distribution throughout the hydrogels with 3 wt.% polymer content, which was demonstrated histologically, immunohistochemically, and by histomorphometric quantification of key ECM components such as aggrecan and collagen II. This homogeneous ECM distribution led to the stiffest hydrogels with a 170-fold increase in Young’s modulus over time. In contrast, higher concentrated constructs showed only pericellular matrix deposition resulting in lower construct stiffness. Furthermore, GPC measurements of the supernatant revealed 40% release of unbound hmHA from the low concentrated constructs, but only 5% from high concentrated ones, suggesting that release of hmHA generated porous constructs facilitating ECM distribution throughout the gels. The study contributes to rational and effective bioink development, demonstrating dual function of a supplement enabling bioprinting and at the same time improving biological properties of the resulting constructs.

Speaker:
Julia Hauptstein
University of Würzburg
Additional Authors:
  • Michael Bartolf-Kopp
    University of Würzburg
  • Leonard Forster
    University of Würzburg
  • Dr. Rainer Detsch
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Dr. Tomasz Jüngst
    University of Würzburg
  • Prof. Dr. Jürgen Groll
    University of Würzburg
  • Dr. Jörg Teßmar
    University of Würzburg
  • Prof. Dr. Torsten Blunk
    University of Würzburg