Scientific Programme

Back to overview

Special Poster Session Biofabrication

Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition

Tuesday (17.03.2020)
18:04 - 18:07
Part of:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication

As a promising biofabrication technology, extrusion-based bioprinting has gained significant attention in the last decade. However, materials that offer an "ideal" combination of printability, shape fidelity, suitable crosslinking mechanism and cytocompatibility are rare. Poly(2-oxazoline)s based biomaterials have been discussed as a potential alternative to the more established polyethylene glycol biomaterial system, due to their facile synthesis, physicochemical versatility and good cyto-/biocompatibility. Although a few different POx-based block copolymers that undergo thermogelation were described recently, it's not possible to print the real 3D constructs at this point. Herein, we describe a hybrid system of nanoclay and a thermoresponsive hydrogel as a novel bioink, and discuss its critical properties relevant for extrusion-based bioprinting, including viscoelastic properties, printability and cytocompatibility. The hybrid hydrogel retains its thermogelling properties but is strengthened by the added clay (G’= 5 kPa). Importantly, the shear-thinning character is further enhanced, which, in combination with very rapid structure recovery (~2 s) is highly beneficial for 3D printing. Accordingly, various 3D patterns are printed with high-resolution and greatly enhanced shape fidelity compared to the bioink without added clay. Results from in vitro cytocompatibility and cell-laden printing suggests its feasibility for cell printing. Overall, a novel ideal bioink is proposed in this study which will expand the options available to researchers in 3D bioprinting biofabrication, and lead to more complex and biomimetic structures in the future. In addition, it may provide a new promising material platform for controlled delivery and release, sacrificial material templates in fabrication of microfluidic devices and thermoresponsive self-protection.

Ph.D. Chen Hu
University of Würzburg
Additional Authors:
  • Prof. Dr. Robert Luxenhofer
    University of Würzburg