Unfurtunately the monastery Irsee has been closed by the Bavarian government due to the corona virus.

Therefore we have decided to offer the conference as a web conference only.

In those unpredictable times, protecting our participants’ health has the highest priority for us!

The scientific exchange must not come to a complete standstill and we believe that with this web solution we have found a way to keep the scientific community going.

In order to participate in the livestream of the conference as easy as possible and to give your presentation, we recommend the use of the web browsers Google Chrome or Microsoft Edge.

Here you can find detailed instructions for using the livestream.

As a participant of the Bioinspired 2020 you have received an e-mail containing the login data for the web conference.

The login data for the sessions are provided daily.

In order to allow all conference participants access to the posters, we ask you to upload your poster by login on the conference homepage and clicking on the button "My Submission" in the upper right corner of the conference homepage. Then select the submission to which you want to upload the poster and upload the poster at the bottom of the page.

Furthermore we would like to ask all poster authors to prepare 4 PowerPoint slides to present your poster to the audience.
Please also include your contact details on the digital poster to allow participants to reach you with questions.

Please note that your poster will be pictured publically.

As poster author you can upload your poster similar to the way you submitted your abstract.
Click on "My Submissions" in the upper right corner at the homepage.

Poster documents can be found by opening the respective abstract in the online programme.
If a poster document has already been uploaded for the abstract, it can then be opened and downloaded.

Please note that your poster will be pictured publically.

You can ask your questions via chat already during the presentations!
Please use for this the Q&A (F&A) button!

For further scientific exchange we implemented a discussion forum on the homepage of the DGM.
Please visit and use your DGM or Bioinspired user credentials to login.

Back to overview

Special Poster Session Biofabrication

3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells

Tuesday (17.03.2020)
18:07 - 18:10

One of the fundamental need in tissue engineering to fabricate tissues/organs is the proper vascularization that ensures a sufficient blood supply of tissues with nutrients and oxygen. So far, the in vitro generation of hierarchically organized functional vascular networks has not been achieved yet.

Aim of the project is to biofabricate hierarchically organized vascular structures with the typical three-layered in large and middle sized vessels and two layered wall structure in vessels of microcirculation using vascular wall-resident stem cells (VW-SCs) or human induced pluripotent cells (hiPSCs) that were pre-differentiated into mesodermal progenitor-like cells. Both VW-SCs and induced mesodermal progenitor cells have the capacity to deliver mature cell types that are needed for morphogenesis of the vascular wall such as endothelial cells, smooth muscle cells, pericytes as well as fibroblasts or mesenchymal cells(1). Second essential biofabrication components are advanced printing techniques and matrix material e.g. hydrogels. To bioprint the vascular structures we used two potential approaches, namely standard extrusion and core-shell printing. Bioink was prepared by mixing hydrogel with cells and was bioprinted by using the two approaches. Hydrogel was also supplemented with extracellular matrix components such as Collagen I, Collagen IV or Laminin to support cellular activities and morphogenetic events.

After optimization the printing parameters, cell numbers and cell types, printed bioink were cultured within differentiation medium over up to several weeks under adding VEGF to the culture. In the fallowing day of culturing, especially within collagen-I supplemented hydrogel; cell diffusion and cell elongation were observed. It was determined that cell proliferation, interactions and differentiation depend on the number of initially printed cells. The cells survived within the gel for weeks, entered differentiation and morphogenesis resulting in formation of cell cords after bioprinting. In-depth analyses revealed that the formation of vessel-like structures with lumen formation.

Dr. Leyla Dogan
University of Würzburg
Additional Authors:
  • Ruben G. Scheuring
    University Hospital Würzburg
  • Prof. Dr. Jürgen Groll
    University Hospital Würzburg
  • Prof. Dr. Süleyman Ergün
    University of Würzburg