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Special Poster Session Biofabrication

3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells

Tuesday (17.03.2020)
18:07 - 18:10
Part of:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication

One of the fundamental need in tissue engineering to fabricate tissues/organs is the proper vascularization that ensures a sufficient blood supply of tissues with nutrients and oxygen. So far, the in vitro generation of hierarchically organized functional vascular networks has not been achieved yet.

Aim of the project is to biofabricate hierarchically organized vascular structures with the typical three-layered in large and middle sized vessels and two layered wall structure in vessels of microcirculation using vascular wall-resident stem cells (VW-SCs) or human induced pluripotent cells (hiPSCs) that were pre-differentiated into mesodermal progenitor-like cells. Both VW-SCs and induced mesodermal progenitor cells have the capacity to deliver mature cell types that are needed for morphogenesis of the vascular wall such as endothelial cells, smooth muscle cells, pericytes as well as fibroblasts or mesenchymal cells(1). Second essential biofabrication components are advanced printing techniques and matrix material e.g. hydrogels. To bioprint the vascular structures we used two potential approaches, namely standard extrusion and core-shell printing. Bioink was prepared by mixing hydrogel with cells and was bioprinted by using the two approaches. Hydrogel was also supplemented with extracellular matrix components such as Collagen I, Collagen IV or Laminin to support cellular activities and morphogenetic events.

After optimization the printing parameters, cell numbers and cell types, printed bioink were cultured within differentiation medium over up to several weeks under adding VEGF to the culture. In the fallowing day of culturing, especially within collagen-I supplemented hydrogel; cell diffusion and cell elongation were observed. It was determined that cell proliferation, interactions and differentiation depend on the number of initially printed cells. The cells survived within the gel for weeks, entered differentiation and morphogenesis resulting in formation of cell cords after bioprinting. In-depth analyses revealed that the formation of vessel-like structures with lumen formation.

Dr. Leyla Dogan
University of Würzburg
Additional Authors:
  • Ruben G. Scheuring
    University Hospital Würzburg
  • Prof. Dr. Jürgen Groll
    University Hospital Würzburg
  • Prof. Dr. Süleyman Ergün
    University of Würzburg