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Special Poster Session Biofabrication

Simultaneous printing of skeletal muscle tissue models and customized bioreactor

Tuesday (17.03.2020)
18:10 - 18:13
Part of:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication

Biofabrication of personalized tissues using 3D printing techniques is a new approach which is revolutionizing the field of tissue engineering. Replacement of injured tissues usually faces issues like donor tissue availability and immune rejection of the transplanted tissue. Fabrication and maturation of a functional tissue under in vitro conditions seems possible using bioprinting of the tissue within a printed bioreactor. Combination of these steps will take us one step closer to personalized treatment.

To achieve this, a bioink based on alginate-di-aldehyde (ADA) and gelatin was designed. Schiff’s base bonds between ADA and gelatin as well as temperature dependent crosslinking ensures multilayer bioprintability.

Complexity and maturation of artificial tissues are essential for proper tissue function. To ensure this, biological and technical approaches were combined. High density of skeletal muscle cells (C2C12) were mixed with the bioink and prealigned during the printing system under applied shear forces. In addition a customized bioreactor was designed that simulates in vivo-like dynamic flow conditions to the printed muscle construct. Therefore, first a porous, lignin based tissue container was printed. Bioprinting the cell containing bioink was performed inside the printed container. After printing, the tissue container was sealed by a silicone housing and connected to a dynamic media flow system. Maintaining this condition for at least one week has shown cell proliferation and tissue maturation.


Dipl.-Ing. Claudia Müller
University of Bayreuth
Additional Authors:
  • Marius Gensler
    University Hospital Würzburg
  • Susanne Heid
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Aldo R. Boccaccini
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Dr. Rainer Detsch
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Jan Hansmann
    University Hospital Würzburg
  • Dr. Sahar Salehi
    Universität Bayreuth