Special Poster Session Biofabrication
Biofabrication of personalized tissues using 3D printing techniques is a new approach which is revolutionizing the field of tissue engineering. Replacement of injured tissues usually faces issues like donor tissue availability and immune rejection of the transplanted tissue. Fabrication and maturation of a functional tissue under in vitro conditions seems possible using bioprinting of the tissue within a printed bioreactor. Combination of these steps will take us one step closer to personalized treatment.
To achieve this, a bioink based on alginate-di-aldehyde (ADA) and gelatin was designed. Schiff’s base bonds between ADA and gelatin as well as temperature dependent crosslinking ensures multilayer bioprintability.
Complexity and maturation of artificial tissues are essential for proper tissue function. To ensure this, biological and technical approaches were combined. High density of skeletal muscle cells (C2C12) were mixed with the bioink and prealigned during the printing system under applied shear forces. In addition a customized bioreactor was designed that simulates in vivo-like dynamic flow conditions to the printed muscle construct. Therefore, first a porous, lignin based tissue container was printed. Bioprinting the cell containing bioink was performed inside the printed container. After printing, the tissue container was sealed by a silicone housing and connected to a dynamic media flow system. Maintaining this condition for at least one week has shown cell proliferation and tissue maturation.