Programme

Unfurtunately the monastery Irsee has been closed by the Bavarian government due to the corona virus.

Therefore we have decided to offer the conference as a web conference only.

In those unpredictable times, protecting our participants’ health has the highest priority for us!

The scientific exchange must not come to a complete standstill and we believe that with this web solution we have found a way to keep the scientific community going.

In order to participate in the livestream of the conference as easy as possible and to give your presentation, we recommend the use of the web browsers Google Chrome or Microsoft Edge.

Here you can find detailed instructions for using the livestream.

As a participant of the Bioinspired 2020 you have received an e-mail containing the login data for the web conference.

The login data for the sessions are provided daily.

In order to allow all conference participants access to the posters, we ask you to upload your poster by login on the conference homepage and clicking on the button "My Submission" in the upper right corner of the conference homepage. Then select the submission to which you want to upload the poster and upload the poster at the bottom of the page.

Furthermore we would like to ask all poster authors to prepare 4 PowerPoint slides to present your poster to the audience.
Please also include your contact details on the digital poster to allow participants to reach you with questions.

Please note that your poster will be pictured publically.

As poster author you can upload your poster similar to the way you submitted your abstract.
Click on "My Submissions" in the upper right corner at the homepage.

Poster documents can be found by opening the respective abstract in the online programme.
If a poster document has already been uploaded for the abstract, it can then be opened and downloaded.

Please note that your poster will be pictured publically.

You can ask your questions via chat already during the presentations!
Please use for this the Q&A (F&A) button!

For further scientific exchange we implemented a discussion forum on the homepage of the DGM.
Please visit discussion.dgm.de and use your DGM or Bioinspired user credentials to login.

Back to overview

Special Poster Session Biofabrication

Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D

Tuesday (17.03.2020)
18:13 - 18:16

Natural biological tissue usually contains a 3-dimensional architecture that is comprised of different cell types and structures of intracellular and extracellular matrix. Most importantly, in these well-optimized systems, defined function requires defined structure on macro- as well as microscopic levels. Biofabrication attempts to reproduce 3D structures of natural tissues by a variety of modern fabrication techniques, the foremost being 3D-Printing. However, a deep understanding of the target tissue template, ranging from cell population type to extra- and intracellular matrix structure, is required to allow for biomimetic replicate of tissue form and function.

A variety of microscopy techniques is available to answer the questions of template structure and construct quality, however, most commonly applied techniques have several drawbacks (e.g. removal of water, necessity of external dyes, photon scattering, low optical resolution) that complicate 3D imaging of living samples or may even alter sample structure due to light-matter interactions.

We use Multi-Photon Excitation Microscopy as a technique with the potential to provide in-depth tissue template analysis as well as quality control of biofabricated constructs.

This results in two major advantages of the system:

- Long wavelength light may be used to excite fluorochromes. This allows for the exploitation of the near infrared (NIR) optical window in biological tissue for excitation, thereby reducing absorption and scattering and effectively increasing penetration depth.

- The signal emission occurs confined in all three spatial dimensions as the signal intensity If is proportional to the excitation light intensity Iex to the power of n, with n being the number of interacting photons (If ∝ (Iex)n). Thus, only very high intensity illumination in the focal spot produces a signal.

- Availability of additional contrast caused by elastic optically nonlinear frequency multiplication effects, such as Second- or Third-Harmonic-Generation (SHG, THG), that are produced intrinsically by certain biopolymer structures, such as fibrous collagen I or myosin II.

Speaker:
Dipl.-Ing. Dominik Schneidereit
Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Additional Authors:
  • Stefanie Diermeier
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Birgitta Carlé
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Anita Bröllochs
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Dr. Sebastian Schürmann
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Oliver Friedrich
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)

Dateien

Category Short file description File description File Size
Poster Poster Version 9;now containing contact email, minor revision MULTIPHOTON MICROSCOPY: A POWERFUL TOOL TO REVEAL CELLULAR ORGANIZATION AND MORPHOLOGY WITHIN BIOENGINEERED CONSTRUCTS IN 3D 4 MB Download