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Special Poster Session Biofabrication

Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D

Tuesday (17.03.2020)
18:13 - 18:16
Part of:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication

Natural biological tissue usually contains a 3-dimensional architecture that is comprised of different cell types and structures of intracellular and extracellular matrix. Most importantly, in these well-optimized systems, defined function requires defined structure on macro- as well as microscopic levels. Biofabrication attempts to reproduce 3D structures of natural tissues by a variety of modern fabrication techniques, the foremost being 3D-Printing. However, a deep understanding of the target tissue template, ranging from cell population type to extra- and intracellular matrix structure, is required to allow for biomimetic replicate of tissue form and function.

A variety of microscopy techniques is available to answer the questions of template structure and construct quality, however, most commonly applied techniques have several drawbacks (e.g. removal of water, necessity of external dyes, photon scattering, low optical resolution) that complicate 3D imaging of living samples or may even alter sample structure due to light-matter interactions.

We use Multi-Photon Excitation Microscopy as a technique with the potential to provide in-depth tissue template analysis as well as quality control of biofabricated constructs.

This results in two major advantages of the system:

- Long wavelength light may be used to excite fluorochromes. This allows for the exploitation of the near infrared (NIR) optical window in biological tissue for excitation, thereby reducing absorption and scattering and effectively increasing penetration depth.

- The signal emission occurs confined in all three spatial dimensions as the signal intensity If is proportional to the excitation light intensity Iex to the power of n, with n being the number of interacting photons (If ∝ (Iex)n). Thus, only very high intensity illumination in the focal spot produces a signal.

- Availability of additional contrast caused by elastic optically nonlinear frequency multiplication effects, such as Second- or Third-Harmonic-Generation (SHG, THG), that are produced intrinsically by certain biopolymer structures, such as fibrous collagen I or myosin II.

Dipl.-Ing. Dominik Schneidereit
Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Additional Authors:
  • Stefanie Diermeier
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Birgitta Carlé
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Anita Bröllochs
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Dr. Sebastian Schürmann
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Oliver Friedrich
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)