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Special Poster Session Biofabrication

Evaluation of inkjet printing for ADA-PEG bioinks

Tuesday (17.03.2020)
18:16 - 18:19
Part of:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication

Evaluation of inkjet printing for ADA-PEG bioinks

Emine Karakaya, Andreas Frank, Leo Forster, Jörg Teßmar,

Hans-Werner Schmidt, Aldo Boccaccini & Rainer Detsch

Inkjet printing of living cells is an emerging additive manufacturing (AM) technology for different life science approaches, for example regenerative medicine, cancer research, lab on a chip and synthetic biology. It is well known that piezo inkjet technology is successfully applied to water-based bioink systems [1]. For this technology, sodium alginate is one favorable bioink because of its high biocompatibility. Additionally, synthetic polymers like poly(ethylene glycol) (PEG) are promising candidates to further tune the properties to improve the final properties of the bioink.

The inkjet printing applied in this study is based on an electromagnetic valve and is able to handle higher viscosities compared to the piezo technology. Regarding the bioinks, we developed a set of hybrid materials consisting of alginate and star-shaped PEGs (star-PEGs) that can be independently varied in physical characteristics e.g. molecular weight and biomolecular functionalization. Alginate polymers were oxidized to alginate dialdehyde (ADA) [2] to provide reaction sites for modified PEG compounds (e.g. amino-PEG). In order to characterize the PEG-modified bioinks and to monitor the success of the reaction between ADA and Amino-PEGs, fourier-transform infrared spectroscopy and ultraviolet–visible spectroscopy were used, respectively. Viscosity and gelation were investigated using rheology measurements. Finally, we embedded NIH3T3-cells in ADA-PEG hydrogels in order to use this combination as a suitable bioink for drop-on-demand approaches. The cell behavior were analysed after 24 h of incubation.

In summary, it has been proven that the inkjet printing process of cells in different ADA-PEG hydrogel formulations is a promising approach. The applied electromagnetic inkjet printing is shown to be completely programmable, accurate and the resolution of the device allowed printing of various patterns with bioinks and vital cells.

[1] Detsch R, Blob S, Zehnder T, Boccaccini AR. Evaluation of cell inkjet printing technique for biofabrication. BioNanoMaterials. 2016

[2] Sarker B, Papageorgiou DG, Silva R, Zehnder T, Gul-E-Noor F, Bertmer M, et al. Fabrication of alginate-gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties. J Mater Chem B. 2014


Ph.D. Emine Karakaya
Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Additional Authors:
  • Andreas Frank
    University of Bayreuth
  • Leonard Forster
    University of Würzburg
  • Prof. Dr. Hans-Werner Schmidt
    University of Bayreuth
  • Dr. Jörg Teßmar
    University of Würzburg
  • Prof. Dr. Aldo R. Boccaccini
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Dr. Rainer Detsch
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)