Unfurtunately the monastery Irsee has been closed by the Bavarian government due to the corona virus.

Therefore we have decided to offer the conference as a web conference only.

In those unpredictable times, protecting our participants’ health has the highest priority for us!

The scientific exchange must not come to a complete standstill and we believe that with this web solution we have found a way to keep the scientific community going.

In order to participate in the livestream of the conference as easy as possible and to give your presentation, we recommend the use of the web browsers Google Chrome or Microsoft Edge.

Here you can find detailed instructions for using the livestream.

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Back to overview

Special Poster Session Biofabrication

Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container

Tuesday (17.03.2020)
18:19 - 18:22

Introduction: Targeted therapies with biologicals are of growing clinical importance in the treatment of autoimmune diseases or cancer. Although auspicious, there are limitations in the broad medical application: the production of biologicals is complex and expensive and the serum half-life time of some biologicals is short requiring frequent injections or infusions. The generation of a drug-producing tissue container vascularized by an AV loop and implanted in the patient seems to be a promising approach to solve this problem. This study aimed at the establishment of an ideal carrier matrix based on recombinant engineered spider silk for the generation of a transplantable therapeutic tissue container.

Materials and methods: For cytocompatibility and drug release testing in vitro, genetically modified HEK293 cells, producing a reporter molecule consisting of the extracellular domain of TNFR2 linked to luciferase, were incorporated into recombinant engineered ADF4(C16)-RGD spider silk. Furthermore, biocompatibility and angiogenesis were assessed in the rat AV loop model using µCT and histology.

Results: Modifying the eADF4(C16) spider silk with the RGD motive supported cell viability as well as the production of the reporter molecule. Moreover, we were able to prove a slow biodegradation and incipient vascularization of constructs containing eADF4(C16)-RGD spider silk. Although, macrophages were present in the histological specimen no signs of severe immunoreaction were observed.

Conclusion: This study demonstrates the impact of modifying engineered ADF4(C16) spider silk with functional groups on cytocompatibility, protein production as well as angiogenesis. The use of engineered ADF4(C16)-RGD spider silk enables new possibilities for the establishment of a transplantable therapeutic tissue container.

Vanessa Trossmann
University of Bayreuth
Additional Authors:
  • Stefanie Heltmann-Meyer
    Universitätsklinikum Erlangen
  • Sophie Winkler
    Universitätsklinikum Erlangen
  • Hanna Amouei
    University Hospital Würzburg
  • Harald Wajant
    University Hospital Würzburg
  • Dr.-Ing. Tobias Fey
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Peter Greil
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Thomas Scheibel
    Universität Bayreuth
  • Andreas Arkudas
    Universitätsklinikum Erlangen
  • Raymund E. Horch
    Universitätsklinikum Erlangen
  • Dr. Dominik Steiner
    Universitätsklinikum Erlangen