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Special Poster Session Biofabrication

Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container

Tuesday (17.03.2020)
18:19 - 18:22
Part of:
17:40 Special Poster Session Biofabrication Hyaluronan based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells 1 Leonard Forster
17:43 Special Poster Session Biofabrication Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks 1 Ilona Paulus
17:46 Special Poster Session Biofabrication Poly(2-oxazoline)/poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations 1 Lukas Hahn
17:49 Special Poster Session Biofabrication Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes 1 Stephan Altmann
17:52 Special Poster Session Biofabrication Fiber reinforced hydrogels – a new platform technology in biofabrication 1 Dipl.-Ing. David Sonnleitner
17:55 Special Poster Session Biofabrication 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks 1 Hannes Horder
17:58 Special Poster Session Biofabrication Hydrogels based on (AB)n-segmented copolymers with polyethylene glycol segments for biofabrication 1 Andreas Frank
18:01 Special Poster Session Biofabrication Metabolic glycoengineering and bioinks 1 Jürgen Mut
18:04 Special Poster Session Biofabrication Improved Printability of a Novel Thermoresponsive Hydrogel Bioink by Nanoclay Addition 1 Ph.D. Chen Hu
18:07 Special Poster Session Biofabrication 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells 1 Dr. Leyla Dogan
18:10 Special Poster Session Biofabrication Simultaneous printing of skeletal muscle tissue models and customized bioreactor 1 Dipl.-Ing. Claudia Müller
18:13 Special Poster Session Biofabrication Multiphoton Microscopy: A Powerful Tool to Reveal Cellular Organization and Morphollogy within Bioengineered Constructs in 3D 1 Dipl.-Ing. Dominik Schneidereit
18:16 Special Poster Session Biofabrication Evaluation of inkjet printing for ADA-PEG bioinks 1 Ph.D. Emine Karakaya
18:19 Special Poster Session Biofabrication Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container 1 Dr. Dominik Steiner
18:22 Special Poster Session Biofabrication 4D Biofabrication of Skeletal Muscle Microtissue Using Electrospun Bilayers 2 Indra Apsite

Session S.1: Special Poster Session Biofabrication Session 1
Belongs to:
General Topic S: Special Poster Session Biofabrication

Introduction: Targeted therapies with biologicals are of growing clinical importance in the treatment of autoimmune diseases or cancer. Although auspicious, there are limitations in the broad medical application: the production of biologicals is complex and expensive and the serum half-life time of some biologicals is short requiring frequent injections or infusions. The generation of a drug-producing tissue container vascularized by an AV loop and implanted in the patient seems to be a promising approach to solve this problem. This study aimed at the establishment of an ideal carrier matrix based on recombinant engineered spider silk for the generation of a transplantable therapeutic tissue container.

Materials and methods: For cytocompatibility and drug release testing in vitro, genetically modified HEK293 cells, producing a reporter molecule consisting of the extracellular domain of TNFR2 linked to luciferase, were incorporated into recombinant engineered ADF4(C16)-RGD spider silk. Furthermore, biocompatibility and angiogenesis were assessed in the rat AV loop model using µCT and histology.

Results: Modifying the eADF4(C16) spider silk with the RGD motive supported cell viability as well as the production of the reporter molecule. Moreover, we were able to prove a slow biodegradation and incipient vascularization of constructs containing eADF4(C16)-RGD spider silk. Although, macrophages were present in the histological specimen no signs of severe immunoreaction were observed.

Conclusion: This study demonstrates the impact of modifying engineered ADF4(C16) spider silk with functional groups on cytocompatibility, protein production as well as angiogenesis. The use of engineered ADF4(C16)-RGD spider silk enables new possibilities for the establishment of a transplantable therapeutic tissue container.

Dr. Dominik Steiner
Universitätsklinikum Erlangen
Additional Authors:
  • Stefanie Heltmann-Meyer
    Universitätsklinikum Erlangen
  • Vanessa Trossmann
    Universität Bayreuth
  • Sophie Winkler
    Universitätsklinikum Erlangen
  • Hanna Amouei
    University Hospital Würzburg
  • Harald Wajant
    University Hospital Würzburg
  • Dr.-Ing. Tobias Fey
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Peter Greil
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Thomas Scheibel
    Universität Bayreuth
  • Andreas Arkudas
    Universitätsklinikum Erlangen
  • Raymund E. Horch
    Universitätsklinikum Erlangen