Scientific Programme

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Synthetic building blocks to direct cells

Monday (16.03.2020)
13:10 - 13:40
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We apply polymeric molecular and nano to micron scale building blocks to assemble soft 3D b iomimetic constructs, which allow studying and controlling cell/material interactions 1 . Hybrid artificial biomaterial matrices are created with anisotropic and dynamic properties. Microgels and fibers are produced by adapted technologies based on fiber spi nning, microfluidics, and in mold polymerization. 2 4 To arrange the building blocks in a spatially controlled manner, we rely on self assembly mechanisms and assembly by external magnetic fields. One of the new material platforms we developed is the Anisog el. It offers a solution for the particular challenge of regenerating sensitive tissues with an oriented architecture, which require a low invasive therapy. The Anisogel can be injected as a liquid and structured in situ in a controlled manner with defined biochemical, mechanical, and structural parameter. Magnetoceptive, anisometric microgels or short fibers are incorporated as building blocks to create a unidirectional structure 5 8 . Cells and nerves grow in a linear manner and the fibronectin produced by fibroblasts is aligned. RGD modification of the microgels further improves the orientation of the cells but significantly reduces fibronectin production. The mechano sensitive protein yes associated protein shuttles to the nucleus due to the mechanical ani sotropy of the Anisogel. Regenerated nerves are functional with spontaneous activity and electrical signals propagating along the anisotropy axis of the material. Another developed platform is a thermoresponsive hydrogel system, encapsulated with plasmonic gold nanorods, to control and vary the mechanical properties of the gel in a dynamic and reversible manner using light 9 . This system elucidates how rapid mechanical transitions lead to nuclear translocation of the protein myocardin related transcription f actor A, depending on the ampli tude and frequency of actuation.

1. J. Rose, L. De Laporte. Advanced Healthcare Materials 2018, 7(6):e1701067.
2. L.P.B. Guerzoni, J. Bohl, A. Jans, J.C. Rose, J. Köhler, A.J.C. Kuehne, L. De Laporte L. Biomaterials Science. 2017, 5(8): 1549-57.
3. L.P.B. Guerzoni, A. Jans, D.B. Gehlen, J. Rose, T. Haraszti, Wessling M, Kuehne AJC, De Laporte L. Small 2019, e1900692.
4. L.P.B. Guerzoni, Y. Tsukamoto, M. Akashi, L. De Laporte. A Layer-by-layer single-cell coating technique to produce injectable mini heart tissues via microfluidics. Biomacromolecules. 2019, Available online.
5. J.C. Rose, M. Cámara-Torres, K. Rahimi, J. Köhler, M. Möller, L. De Laporte. Nano Letters, 2017, 17(6): 3782-91.
6. J.C. Rose, D.B. Gehlen, T. Haraszti, J. Köhler, C.J. Licht, L. De Laporte. Biomaterials 2018, 163:128-41.
7. A. Omidinia-Anarkoli, S. Boesveld, U. Tuvshindorj, J. Rose, T. Haraszti, L. De Laporte. Small 2017, 13(36).
8. A. Omidinia-Anarkoli, R. Rimal, Y. Chandorkar, D. Gehlen, J.C. Rose, K. Rahimi, L. De Laporte. ACS Applied Materials and Interfaces 2019, 11(8): 7671-85.
9. Y. Chandorkar, A. Castro Nava, H. Zhang, S. Schweizerhof, M. van Dongen, T. Haraszti, J. Köhler, H. Zhang, R. Windoffer, A. Mourran, M. Möller, L. De Laporte. Nature Communications. 2019 Sep 6;10(1):4027.

Prof. Dr. Laura De Laporte
DWI - Leibniz Institute for Interactive Materials