Additive manufacturing technologies, often referred to as 3D printing, open exciting perspectives for biomedical applications and tissue engineering. They range from producing patient-specific 3D scaffolds, which are subsequently seeded with cells, to direct processing of cell-containing materials in accordance to a computer assisted design (CAD) models, the so-called bioprinting. Among the myriad of possible approaches multiphoton lithography (MPL) stands out as a technique enabling true 3D structuring with spatial resolution down to submicrometer level. The portfolio of biocompatible MPL materials expanded substantially in the recent years. In this contribution the recent progress of MPL development for biomedical applications, will be discussed. The presentation is supported by numerous examples.